MD13
Tocris Bioscience, part of Bio-Techne | Catalog # 7503
Potent MIF Degrader (PROTAC®)
Product Description
MD13 is a potent macrophage migration inhibitory factor (MIF) Degrader (PROTAC®; DC50 < 100 nM; Dmax = 92% after 6 h). MD13 comprises the cereblon ligand Pomalidomide (Cat. No. 6302) joined by a linker to a MIF inhibitor. MD13 reduces MIF levels, arrests cells at the G2/M phase and inhibits proliferation of A549 lung carcinoma cells. Growth of A549 cell 3D spheroids is also inhibited by MD13.Negative control MD15 (Cat. No. 7504) and MIF antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF-289-PB and MAB289.
PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
Licensing Information
Sold with kind permission of Professor Dekker.Product Specifications
Molecular Weight
653.69
Formula
C35H35N5O8
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
8-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)-N-(4-(7-hydroxy-2-oxo-2H-benzo[e][1,3]oxazin-3(4H)-yl)phenyl)octanamide
CAS Number
2758431-97-7
InChI Key
HAHDZDUOFHMMEA-UHFFFAOYSA-N
SMILES
O=C1N(CC2=CC=C(C=C2O1)O)C3=CC=C(C=C3)NC(CCCCCCCNC4=C(C5=CC=C4)C(N(C5=O)C6CCC(NC6=O)=O)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 65.37 | 100 |
Preparing Stock Solutions
for MD13
The following data is based on the product molecular weight 653.69
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.53 mL | 7.65 mL | 15.30 mL |
| 5 mM | 0.31 mL | 1.53 mL | 3.06 mL |
| 10 mM | 0.15 mL | 0.76 mL | 1.53 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.31 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Xiao Proteolysis targeting chimera (PROTAC) for macrophage migration inhibitory factor (MIF) has anti-proliferative activity in lung cancer cells. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34018657
Product Specific Notices for MD13
For research use only
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