Janelia Fluor^®585, Maleimide New

Name: Janelia Fluor ® 585, Maleimide
Brand: Tocris Bioscience
SKU: 8026

Tocris Bioscience, part of Bio-Techne | Catalog # 8026

COA / SDS

Orange dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Key Product Details

Description:	Orange dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name:	2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-10,10-dimethyl-3,10-dihydroanthracen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate
Purity:	≥95% (HPLC)
Molecular Weight:	674.65

View Full Technical Details for Janelia Fluor^®585, Maleimide

Technical Data
Citations

Biological Activity for Janelia Fluor^®585, Maleimide

Key information: Janelia Fluor^® 585, Maleimide is an orange fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M^-1cm^-1(measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (K_L-Z) < 0.0001.

Please see the protocol for further information and a guide to protein/antibody labeling.

Janelia Fluor^® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor^®585, Maleimide

Emission Color	Orange
λ_abs	585 nm
λ_em	609 nm
Extinction Coefficient (ε)	156,000 M^-1cm^-1
Quantum Yield (φ)	0.78
Reactive Group	Maleimide
Reactivity	Thiols
Cell Permeable	Yes
Correction Factor 280	0.099
Application	Confocal Imaging, dSTORM, STED, Flow Cytometry

Technical Data for Janelia Fluor^®585, Maleimide

M. Wt	674.65
Formula	C₃₆H₃₀F₄N₄O₅
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	CNCYDQGRLJHJCO-UHFFFAOYSA-N
Smiles	O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Janelia Fluor^®585, Maleimide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	6.75	10

Preparing Stock Solutions for Janelia Fluor^®585, Maleimide

The following data is based on the product molecular weight 674.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	14.82 mL	74.11 mL	148.23 mL
0.5 mM	2.96 mL	14.82 mL	29.65 mL
1 mM	1.48 mL	7.41 mL	14.82 mL
5 mM	0.30 mL	1.48 mL	2.96 mL

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References for Janelia Fluor^®585, Maleimide

References are publications that support the biological activity of the product.

Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
Grimm A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 2015 PMID: 25599551

Janelia Fluor^®585, Maleimide New

Tocris Bioscience, part of Bio-Techne | Catalog # 8026

Key Product Details

Biological Activity for Janelia Fluor^®585, Maleimide

Licensing Information

Optical Data for Janelia Fluor^®585, Maleimide

Technical Data for Janelia Fluor^®585, Maleimide

Solubility for Janelia Fluor^®585, Maleimide

Preparing Stock Solutions for Janelia Fluor^®585, Maleimide

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for Janelia Fluor^®585, Maleimide

Product Documents for Janelia Fluor^®585, Maleimide

COA

Certificate of Analysis

SDS

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Janelia Fluor® 585, Maleimide New

Tocris Bioscience, part of Bio-Techne | Catalog # 8026

Key Product Details

Biological Activity for Janelia Fluor® 585, Maleimide

Licensing Information

Optical Data for Janelia Fluor® 585, Maleimide

Technical Data for Janelia Fluor® 585, Maleimide

Solubility for Janelia Fluor® 585, Maleimide

Preparing Stock Solutions for Janelia Fluor® 585, Maleimide

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for Janelia Fluor® 585, Maleimide

Product Documents for Janelia Fluor® 585, Maleimide

COA

Certificate of Analysis

SDS

Janelia Fluor^®585, Maleimide New

Biological Activity for Janelia Fluor^®585, Maleimide

Optical Data for Janelia Fluor^®585, Maleimide

Technical Data for Janelia Fluor^®585, Maleimide

Solubility for Janelia Fluor^®585, Maleimide

Preparing Stock Solutions for Janelia Fluor^®585, Maleimide

References for Janelia Fluor^®585, Maleimide

Product Documents for Janelia Fluor^®585, Maleimide