Hesperadin hydrochloride

Name: Hesperadin hydrochloride
Brand: Tocris Bioscience
SKU: 3988

Tocris Bioscience, part of Bio-Techne | Catalog # 3988

Citations (9)

COA / SDS

Potent Aurora kinase B inhibitor

Key Product Details

Description:	Potent Aurora kinase B inhibitor
Chemical Name:	N-[2,3-Dihydro-2-oxo-3-[(3Z)-phenyl[[4-(1-piperidinylmethyl)phenyl]amino]methylene]-1H-indol-5-yl]-ethanesulfonamide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	553.12

View Full Technical Details for Hesperadin hydrochloride

Technical Data
Citations

Biological Activity for Hesperadin hydrochloride

Hesperadin hydrochloride is an ATP-competitive inhibitor of Aurora B kinase (IC₅₀ = 250 nM). Prevents chromosome alignment and segregation; also induces polyploidy and prevents histone H3-Ser10 phosphorylation. Overrides the spindle assembly checkpoint and induces mitotic exit in monastrol- and taxol-treated HeLa cells. Inhibits replication of influenza A and B viral strains (IC₅₀ values range from 0.22 μM to 1.8 μM, dependant on strain), by inhibiting viral RNA transcription and translation

Compound Libraries for Hesperadin hydrochloride

Hesperadin hydrochloride is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for Hesperadin hydrochloride

M. Wt	553.12
Formula	C₂₉H₃₂N₄O₃S.HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
PubChem ID	76968689
InChi Key	XMYLEXZLFZAOQN-LXCLTORNSA-N
Smiles	O=C3/C(C2=CC(NS(CC)(=O)=O)=CC=C2N3)=C(C4=CC=CC=C4)\NC1=CC=C(CN5CCCCC5)C=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hesperadin hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	55.31	100
	ethanol	27.66	50

Preparing Stock Solutions for Hesperadin hydrochloride

The following data is based on the product molecular weight 553.12.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.81 mL	9.04 mL	18.08 mL
5 mM	0.36 mL	1.81 mL	3.62 mL
10 mM	0.18 mL	0.90 mL	1.81 mL
50 mM	0.04 mL	0.18 mL	0.36 mL

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References for Hesperadin hydrochloride

References are publications that support the biological activity of the product.

Hu Chemical genomics approach leads to the identification of hesperadin, an aurora B kinase inhibitor, as a broad-spectrum influenza antiviral. Int.J.Mol.Sci. 2017 PMID: 28885544
Jetton The cell cycle as a therapeutic target against Trypanosoma brucei: Hesperadin inhibits Aurora kinase-1 and blocks mitotic progression in bloodstream forms. Mol.Microbiol. 2009 PMID: 19320832
Sessa Mechanism of Aurora B activation by INCENP and inhibition by Hesperadin. Mol.Cell. 2005 PMID: 15866179
Hauf The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint. J.Cell.Biol. 2003 PMID: 12707311