EGCG is a β-secretase (BACE1) inhibitor (IC₅₀ = 1.6 μM); inhibits amyloid assembly. Inhibits the formation of amyloid fibrils by proteins such as Aβ, α-synuclein and huntingtin. Reduces Aβ42-induced cell death in three different cell types. Also inhibits glutamate dehydrogenase. Inhibits cell growth and selectively induces apoptosis in A431 and human pancreatic cancer cells. Also a DNMT1 inhibitor.

Product Specifications

Molecular Weight

458.37

Formula

C₂₂H₁₈O₁₁

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3,4,5-Trihydroxybenzoic acid (2R,3R)-3,4-dihydro-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-2H-1-benzopyran-3-yl ester

CAS Number

989-51-5

PubChem ID

65064

InChI Key

WMBWREPUVVBILR-WIYYLYMNSA-N

SMILES

O=C(C4=CC(O)=C(O)C(O)=C4)O[C@@H]2CC1=C(O)C=C(O)C=C1O[C@@H]2[C@]3=CC(O)=C(O)C(O)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	4.58	10
	DMSO	45.84	100

Preparing Stock Solutions for EGCG

The following data is based on the product molecular weight 458.37

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.91 mL	21.82 mL
5 mM	0.44 mL	2.18 mL	4.36 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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Background References

References are publications that support the biological activity of the product.

Yang Glioblastoma cells require glutamate dehydrogenase to survive impairments of glucose metabolism or Akt signaling. Cancer Res. 2009 PMID: 19826036
Lee Mechanisms for the inhibition of DNA methyltransferases by tea catechins and bioflavonoids. Mol.Pharmacol. 2005 PMID: 16037419
Shankar EGCG inhibits growth, invasion, angiogenesis and metastastasis of pancreatic cancer. Front.Biosci. 2008 PMID: 17981559
Ahmad Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor κB in cancer cells versus normal cells. Ach.Biochem.Biophys. 2000 PMID: 10775421
Sinha Comparison of three amyloid assembly inhibitors: the sugar scyllo-inositol, the polyphenol epigallocatechin gallate, and the molecular tweezer CLR01. ACS Chem.Neurosci. 2012 PMID: 22860214
Jeon Green tea catechins as a BACE1 (β-secretase) inhibitor. Bioorg.Med.Chem.Lett. 2003 PMID: 14592472

EGCG

Tocris Bioscience, part of Bio-Techne | Catalog # 4524

Key Product Details

Description

Alternative Names

View all Amyloid beta Peptide Inhibitors »

Product Description