dTAGV-1
Tocris Bioscience, part of Bio-Techne | Catalog # 6914
Potent and selective degrader of mutant FKBP12F36V fusion proteins
Key Product Details
Description
Potent and selective degrader of mutant FKBP12F36V fusion proteins
Product Description
dTAGV-1 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a von Hippel-Lindau (VHL)-binding ligand. Induces potent and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. Selectively degrades FKBP12F36V-EWS/FLI fusion proteins and inhibits cell proliferation in FKBP12F36V-EWS/FLI-expressing Ewing sarcoma cells.Hydrochloride salt (Cat.No. 7374) available; suitable for in vivo use.
Negative control dTAGV-1-NEG (Cat. No. 6915) also available.
FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.
Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.
Licensing Information
Sold under license from Dana-Farber Cancer InstituteProduct Specifications
Molecular Weight
1361.58
Formula
C68H90N6O14S CF3CO2H
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((7-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-7-oxoheptyl)amino)-2-oxoethoxy)phenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate trifluoroacetate
CAS Number
2624313-15-9
InChI Key
KSEWNBIDXKMTNT-LNVAYBNASA-N
SMILES
C[C@H](NC([C@@H]1C[C@@H](O)CN1C([C@@H](NC(CCCCCCNC(COC2=CC=CC=C2[C@H](OC([C@@H]3CCCCN3C([C@@H](CC)C4=CC(OC)=C(OC)C(OC)=C4)=O)=O)CCC5=CC(OC)=C(OC)C=C5)=O)=O)C(C)(C)C)=O)=O)C6=CC=C(C=C6)C7=C(N=CS7)C.OC(C(F)(F)F)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 136.16 | 100 |
Preparing Stock Solutions
for dTAGV-1
The following data is based on the product molecular weight 1361.58
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.73 mL | 3.67 mL | 7.34 mL |
| 5 mM | 0.15 mL | 0.73 mL | 1.47 mL |
| 10 mM | 0.07 mL | 0.37 mL | 0.73 mL |
| 50 mM | 0.01 mL | 0.07 mL | 0.15 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Abuhashem Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system. STAR Protoc. 2022 PMID: 36097386
- Nabet Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat.Commun. 2020 PMID: 32948771
Product Specific Notices for dTAGV-1
For research use only
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