Dorsomorphin dihydrochloride

Product Description

Dorsomorphin dihydrochloride is a potent inhibitor of AMP-activated protein kinase (AMPK) (K_i = 109 nM). Displays no significant activity on several structurally related kinases including ZAPK, SYK, PKCθ, PKA and JAK3. Inhibits AMPK activation induced by AICAR (Cat. No. 2840) and Metformin (Cat. No. 2864). Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs.Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition. Also used in a protocol to generate brain organoids from human iPSCs.

Solubility

Preparing Stock Solutions for Dorsomorphin dihydrochloride

The following data is based on the product molecular weight 472.41

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.12 mL	10.58 mL	21.17 mL
5 mM	0.42 mL	2.12 mL	4.23 mL
10 mM	0.21 mL	1.06 mL	2.12 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Schafer An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 2023 PMID: 37172564
• Chen Chemically defined neural conversion of human pluripotent stem cells. Methods Mol.Biol. 2019 PMID: 30656621
• Guan Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 2022 PMID: 35418683
• Diekmann A reliable and efficient protocol for human pluripotent stem cell differentiation into the definitive endoderm based on dispersed single cells. Stem Cells Dev. 2015 PMID: 25137387
• Hao Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS ONE 2008 PMID: 18682835
• Vucicevic Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway. Autophagy 2011 PMID: 20980833
• Yu Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nat.Chem.Biol. 2008 PMID: 18026094
• Zhou Role of AMP-activated protein kinase in mechanism of MetF. action. J.Clin.Invest. 2001 PMID: 11602624