AICAR

Tocris Bioscience, part of Bio-Techne | Catalog # 2840

(1)

Citations (138)

COA / SDS

AMPK activator

Key Product Details

Description:	AMPK activator
Chemical Name:	N¹-(β-D-Ribofuranosyl)-5-aminoimidazole-4-carboxamide
Purity:	≥99% (HPLC)
Molecular Weight:	258.23

View Full Technical Details for AICAR

Technical Data
Citations / Reviews (1)

Biological Activity for AICAR

AICAR is a cell-permeable, allosteric activator of AMP-activated protein kinase (AMPK). Augments proliferation, differentiation and mineralization of osteoblastic MC3T3-EI cells and attenuates psychosine-induced expression of proinflammatory cytokines and iNOS in astrocytes. Promotes osteogenic differentiation of hAMSCs and BM-MSCs in vitro.

Compound Libraries for AICAR

AICAR is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for AICAR

M. Wt	258.23
Formula	C₉H₁₄N₄O₅
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	2627-69-2
PubChem ID	17513
InChi Key	RTRQQBHATOEIAF-UUOKFMHZSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC(C(N)=O)=C2N)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AICAR

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	19.37	75
	DMSO	19.37	75

Preparing Stock Solutions for AICAR

The following data is based on the product molecular weight 258.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.75 mM	5.16 mL	25.82 mL	51.63 mL
3.75 mM	1.03 mL	5.16 mL	10.33 mL
7.5 mM	0.52 mL	2.58 mL	5.16 mL
37.5 mM	0.10 mL	0.52 mL	1.03 mL

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Reconstitution Calculator

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References for AICAR

References are publications that support the biological activity of the product.

Wu AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells. Int.J.Artif.Organs. 2011 PMID: 22198598
Kanazawa Adiponectin and AMP kinase activator stimulate proliferation, differentiation and mineralization of osteoblastic MC3T3-EI cells. BMC Cell Biol. 2007 PMID: 18047638
Giri The role of AMPK in psychosine mediated effects on oligodendrocytes and astrocytes: Implication for Krabbe disease. J.Neurochem. 2008 PMID: 18248608
Giri 5-Aminoimidazole-4-carboxamide-1-β-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J.Neurosci. 2004 PMID: 14724246