D 4476

Tocris Bioscience, part of Bio-Techne | Catalog # 2902

COA / SDS

Selective CK1 inhibitor. Also inhibits TGF-βRI

Key Product Details

Description:	Selective CK1 inhibitor. Also inhibits TGF-βRI
Chemical Name:	4-[4-(2,3-Dihydro-1,4-benzodioxin-6-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	398.41

View Full Technical Details for D 4476

Technical Data
Citations

Biological Activity for D 4476

D 4476 is a selective inhibitor of casein kinase 1 (CK1) and TGF-β type-I receptor (ALK5) that displays > 20-fold selectivity over SAPK2/p38 and a much greater selectivity over all other protein kinases tested. Suppresses site-specific phosphorylation and nuclear exclusion of FOXO1a.

Licensing Information

Sold with the permission of GlaxoSmithKline

Compound Libraries for D 4476

D 4476 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for D 4476

M. Wt	398.41
Formula	C₂₃H₁₈N₄O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	301836-43-1
PubChem ID	6419753
InChi Key	DPDZHVCKYBCJHW-UHFFFAOYSA-N
Smiles	O=C(N)C4=CC=C(C=C4)C1=NC(C2=CC(OCCO5)=C5C=C2)=C(C3=NC=CC=C3)N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for D 4476

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	39.84	100
	ethanol	19.92	50

Preparing Stock Solutions for D 4476

The following data is based on the product molecular weight 398.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.51 mL	12.55 mL	25.10 mL
5 mM	0.50 mL	2.51 mL	5.02 mL
10 mM	0.25 mL	1.25 mL	2.51 mL
50 mM	0.05 mL	0.25 mL	0.50 mL

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References for D 4476

References are publications that support the biological activity of the product.

Rena D4476, a cell-permeant inhibitor of CK1, suppresses the site-specific phosphorylation and nuclear exclusion of FOXO1a. EMBO Rep. 2004 PMID: 14710188
Bain The selectivity of protein kinase inhibitors: a further update. Biochem.J. 2007 PMID: 17850214
Callahan Identification of novel inhibitors of the transforming growth factor β1 (TGF-β1) type I receptor (ALK5). J.Med.Chem. 2002 PMID: 11855979