Cycloheximide
Tocris Bioscience, part of Bio-Techne | Catalog # 0970
Inhibitor of protein synthesis
Key Product Details
Description
Inhibitor of protein synthesis
Product Description
Cycloheximide is a selective inhibitor of eukaryotic (over prokaryotic) protein synthesis, blocking tRNA binding and release from ribosomes. Induces apoptosis in a variety of transformed and normal cell lines, including T cells. Competitively inhibits the PPIase hFKBP12 (Ki = 3.4 μM). Also inhibits ferroptosis. Antifungal antibiotic. Exhibits anti-MERS-CoV activity in Vero cells in vitro (IC50 = 0.16 μM).Product Specifications
Molecular Weight
281.35
Formula
C15H23NO4
Storage
Desiccate at RT
Purity
≥97% (HPLC)
Chemical Name
4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione
CAS Number
66-81-9
PubChem ID
6197
InChI Key
YPHMISFOHDHNIV-FSZOTQKASA-N
SMILES
O=C(N1)CC(C[C@@H](O)[C@]2([H])C[C@@H](C)C[C@H](C)C2=O)CC1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 7.03 | 25 | |
| Ethanol | 14.07 | 50 |
Preparing Stock Solutions
for Cycloheximide
The following data is based on the product molecular weight 281.35
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 7.11 mL | 35.54 mL | 71.09 mL |
| 2.5 mM | 1.42 mL | 7.11 mL | 14.22 mL |
| 5 mM | 0.71 mL | 3.55 mL | 7.11 mL |
| 25 mM | 0.14 mL | 0.71 mL | 1.42 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Sawicki and Sawicki Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis. J.Virol. 1986 PMID: 2867230
- Ko Screening of FDA-approved drugs using a MERS-CoV clinical isolate from South Korea identifies potential therapeutic options for COVID-19. Viruses 2021 PMID: 33918958
- Xie Ferroptosis: process and function. Cell.Death.Differ. 2016 PMID: 26794443
- Tang Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism. J.Biol.Chem. 1999 PMID: 10066786
- Obrig The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes. J.Biol.Chem. 1971 PMID: 5541758
- Christner Synthesis and cytotoxic evaluation of cycloheximide derivatives as potential inhibitors of FKBP12 with neuroregenerative properties. J.Med.Chem. 1999 PMID: 10479292
Product Specific Notices for Cycloheximide
For research use only
⚠ WARNING: This product can expose you to chemicals including Cycloheximide, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov
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