CLP 290

Name: CLP 290
Brand: Tocris Bioscience
SKU: 6758

Tocris Bioscience, part of Bio-Techne | Catalog # 6758

COA / SDS

K+/Cl- cotransporter 2 (KCC2) activator; orally bioavailable prodrug of CLP 257 (Cat. No.5242)

Key Product Details

Description:	K⁺/Cl^- cotransporter 2 (KCC2) activator; orally bioavailable prodrug of CLP 257 (Cat. No.5242)
Chemical Name:	(Z)-5-Fluoro-2-((4-oxo-2-(tetrahydropyridazin-1(2H)-yl)thiazol-5(4H)-ylidene)methyl)phenyl pyrrolidine-1-carboxylate
Purity:	≥98% (HPLC)
Molecular Weight:	404.46

View Full Technical Details for CLP 290

Technical Data
Citations

Biological Activity for CLP 290

CLP 290 is a K⁺/Cl^- Cotransporter 2 (KCC2) activator. Activates spinal inhibitory interneurons, restores physiological neuronal activity in the spinal cord and induces functional recovery in a mouse model of spinal cord injury. Also decreases blood vasopressin and glucose levels in mice with Streptozotocin (Cat. No. 1621) induced diabetes mellitus. Orally bioavailable prodrug of CLP 257 (Cat. No.5242).

Compound Libraries for CLP 290

CLP 290 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for CLP 290

M. Wt	404.46
Formula	C₁₉H₂₁FN₄O₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1181083-81-7
PubChem ID	68637267
InChi Key	DIXMMXNNKLCLOM-WJDWOHSUSA-N
Smiles	FC1=CC(OC(N2CCCC2)=O)=C(C=C1)/C=C3SC(N4CCCCN4)=NC\3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CLP 290

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	20.22	50
	ethanol	2.02	5

Preparing Stock Solutions for CLP 290

The following data is based on the product molecular weight 404.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.94 mL	24.72 mL	49.45 mL
2.5 mM	0.99 mL	4.94 mL	9.89 mL
5 mM	0.49 mL	2.47 mL	4.94 mL
25 mM	0.10 mL	0.49 mL	0.99 mL

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Reconstitution Calculator

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References for CLP 290

References are publications that support the biological activity of the product.

Kim Long-term ionic plasticity of GABAergic signalling in the hypothalamus. J.Neuroendocrinol. 2019 PMID: 31166034
Chen Reactivation of dormant relay pathways in injured spinal cord by KCC2 manipulations. Cell 2018 PMID: 30033363
Gagnon Chloride extrusion enhancers as novel therapeutics for neurological diseases. Nat.Med. 2013 PMID: 24097188