ClariTSA™ Fluorophore Kit 690 New
Tocris Bioscience, part of Bio-Techne | Catalog # 9007
Signal amplification kit for use in ISH, ICC and IHC
Key Product Details
Description
Signal amplification kit for use in ISH, ICC and IHC
Product Description
ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 671 nm; emission maximum = 684 nm
Please see the product datasheet or protocol for information on product use.
ClariTSA™ is a trademark of Bio-Techne Corp.
Scientific Data Images for ClariTSA™ Fluorophore Kit 690
Application of ClariTSA™ Fluorophore Kit 690 in RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells.
The target visualised in the image is HPRT1 (red, 1:1750 dilution, 690 channel, ClariTSA™ 690 kit); DAPI (dark blue, Cat. No. 5748) was used as a nuclear counterstain. Imaging was conducted using an Akoya PhenoImager with standardised imaging conditions.
Application of ClariTSA™ Fluorophore Kit 690 in RNAscope™ Multiplex Fluorescent v2 Assay in human colon tissue.
The target visualised in the image is HPRT1 (red, 1:750 dilution, 690 channel, ClariTSA™ 690 kit); DAPI (dark blue, Cat. No. 5748) was used as a nuclear counterstain. Imaging was conducted using an Akoya PhenoImager with standardised imaging conditions. |
Emission Color | Red |
| λabs | 671 nm | |
| λem | 684 nm |
Product Specifications
Storage
-20°C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for ClariTSA™ Fluorophore Kit 690
For research use only
Loading...
Loading...