CEP
Tocris Bioscience, part of Bio-Techne | Catalog # 8108
Chemoproteomic reagent, clickable electrophilic purine for RNA binding profiling in live cells
Key Product Details
Description
Chemoproteomic reagent, clickable electrophilic purine for RNA binding profiling in live cells
Product Description
CEP is a chemoproteomic reagent. It is a clickable electrophilic purine used to directly quantify protein-RNA interactions on proteins through photoaffinity competition with 4-thiouridine (4SU)-labeled RNA in cells. It is a cysteine reactive probe used in photo-activatable-competition and chemoproteomic enrichment (PACCE) method for global quantification of protein-RNA interactions in living cells.Product Specifications
Molecular Weight
227.05
Formula
C8H4Cl2N4
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2,6-Dichloro-7-(2-propyn-1-yl)-7H-purine
CAS Number
1838570-66-3
PubChem ID
155590179
InChI Key
DQZMJDUCMIRFBJ-UHFFFAOYSA-N
SMILES
ClC1=NC(N=CN2CC#C)=C2C(Cl)=N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.7 | 100 | |
| Ethanol | 2.27 | 10 with gentle warming |
Preparing Stock Solutions
for CEP
The following data is based on the product molecular weight 227.05
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.40 mL | 22.02 mL | 44.04 mL |
| 5 mM | 0.88 mL | 4.40 mL | 8.81 mL |
| 10 mM | 0.44 mL | 2.20 mL | 4.40 mL |
| 50 mM | 0.09 mL | 0.44 mL | 0.88 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Heindel Chemoproteomic capture of RNA binding activity in living cells. Nat.Commun. 2023 PMID: 37805600
Product Specific Notices for CEP
For research use only
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