C16

Name: C16
Brand: Tocris Bioscience
SKU: 5382

Tocris Bioscience, part of Bio-Techne | Catalog # 5382

Citations (7)

COA / SDS

PKR inhibitor

Key Product Details

Description:	PKR inhibitor
Chemical Name:	6,8-Dihydro-8-(1H-imidazol-5-ylmethylene)-7H-pyrrolo[2,3-g]benzothiazol-7-one
Purity:	≥98% (HPLC)
Molecular Weight:	268.29

View Full Technical Details for C16

Technical Data
Citations

Biological Activity for C16

C16 is a PKR inhibitor (IC₅₀ = 210 nM). Rescues PKR-dependent translation block in vitro. Decreases Aβ42-induced inflammatory cytokine release and apoptosis in neuronal cultures. Prevents neuroinflammation and neuronal loss in an acute excitotoxic rat model. Brain penetrant.

Technical Data for C16

M. Wt	268.29
Formula	C₁₃H₈N₄OS
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	608512-97-6
PubChem ID	6490494
InChi Key	VFBGXTUGODTSPK-BAQGIRSFSA-N
Smiles	O=C2NC1=CC=C4C(SC=N4)=C1/C2=C/C3=CNC=N3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for C16

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	13.41	50
	ethanol	5.37	20

Preparing Stock Solutions for C16

The following data is based on the product molecular weight 268.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	7.45 mL	37.27 mL	74.55 mL
2.5 mM	1.49 mL	7.45 mL	14.91 mL
5 mM	0.75 mL	3.73 mL	7.45 mL
25 mM	0.15 mL	0.75 mL	1.49 mL

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References for C16

References are publications that support the biological activity of the product.

Tronel The specific PKR inhibitor C16 prevents apoptosis and IL-1 beta production in an acute excitotoxic rat model with a neuroinflammatory component. Neurochem.Int. 2014 PMID: 24211709
Ingrand The oxindole/imidazole derivative C16 reduces in vivo brain PKR activation. FEBS Lett. 2007 PMID: 17761171
Couturier Prevention of the β-amyloid peptide-induced inflammatory process by inhibition of double-stranded RNA-dependent protein kinase in primary murine mixed co-cultures. J.Neuroinflammation 2011 PMID: 21699726
Jammi Small molecule inhibitors of the RNA-dependent protein kinase. Biochem.Biophys.Res.Comm. 2003 PMID: 12890478

C16