Bafilomycin A1
Tocris Bioscience, part of Bio-Techne | Catalog # 1334
H+-ATPase (vacuolar) inhibitor
Product Description
Bafilomycin A1 is a highly potent, selective inhibitor of vacuolar H+-ATPases (IC50 = 0.6 - 1.5 nM in bovine chromaffin granules). Selective for v-ATPase over other ATP hydrolyzing enzymes such as F-ATPases and the H+/K+-ATPase (P-ATPase). Thought to inhibit autophagy either by blocking autophagosome-lysosome fusion (in H4IIE cells), or by blocking lysosomal degradation. Bafilomycin A1 accelerates wound healing in db/db mice. Low dose Bafilomycin A1 attenuates patient-derived CD34+CD19+ leukemia stem cells (LSC) and inhibits LSC proliferation in an animal model. Bafilomycin A1 impairs Zika virus infection in vitro.Product Specifications
Molecular Weight
622.84
Formula
C35H58O9
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
CAS Number
88899-55-2
PubChem ID
72311
InChI Key
WEEFNMFMNMASJY-UHFFFAOYSA-M
SMILES
O[C@H]([C@H](C)/C=C(C)/C=C1OC)[C@@H](C)C/C(C)=C/C=C\[C@H](OC)C([C@@H](C)[C@H]([C@@H]([C@@]2(O)C[C@@H](O)[C@H](C)[C@@H]([C@H](C)C)O2)C)O)OC/1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.98 |
Preparing Stock Solutions
for Bafilomycin A1
The following data is based on the product molecular weight 622.84
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.08 mM | 20.07 mL | 100.35 mL | 200.69 mL |
| 0.4 mM | 4.01 mL | 20.07 mL | 40.14 mL |
| 0.8 mM | 2.01 mL | 10.03 mL | 20.07 mL |
| 4 mM | 0.40 mL | 2.01 mL | 4.01 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Xu Bafilomycin A1 targets patient-derived CD34 + CD19 + leukemia stem cells. Haematologica 2020 PMID: 31097633
- Sabino Bafilomycin A1 and U18666A efficiently impair ZIKV infection in vitro. Viruses 2019 PMID: 31174294
- Wang Bafilomycin A1 accelerates chronic refractory wound healing in db/db mice. Biomed.Res.Int. 2020 PMID: 32714982
- Mauthe Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion. Autophagy 2018 PMID: 29940786
- Fass Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes. J.Biol.Chem. 2006 PMID: 16963441
- Yamamoto Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct.Funct. 1998 PMID: 9639028
- Gagliardi Chemistry and stucture activity relationships of bafilomycin A1, a potent and selective inhibitor of the vacuolar H+-ATPase. Curr.Med.Chem. 1999 PMID: 10519916
Product Specific Notices for Bafilomycin A1
For research use only
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