ASP 7967

Name: ASP 7967
Brand: Tocris Bioscience
SKU: 8803

Tocris Bioscience, part of Bio-Techne | Catalog # 8803

COA / SDS

Ligand for RNA aptamer AC17-4 activating gene expression in aptazime-based riboswitches

Key Product Details

Description:	Ligand for RNA aptamer AC17-4 activating gene expression in aptazime-based riboswitches
Chemical Name:	N²,N⁴-Bis(4-fluorophenyl)-N⁶-(2-pyrimidinylmethyl)-1,3,5-triazine-2,4,6-triamine hemifumarate
Purity:	≥98% (HPLC)
Molecular Weight:	464.44

View Full Technical Details for ASP 7967

Technical Data
Citations

Biological Activity for ASP 7967

ASP 7967 is a ligand for RNA aptamer AC17-4 that activates gene expression in synthetic aptazime-based riboswitches at as low as 5 μM concentration, without relying on exogenous protein factors. ASP 7967 binds aptamer AC17-4 with high affinity in HEK293 cells (Kd = 12 nM; EC₅₀ = 2.3 μM), activating gene expression (>10-fold) in HEK293 cells. ON/OFF ratio of riboswitches activated by ASP 7967 approaches 300, with a low basal expression level in cultured cells.

In vivo, ASP 7967 upregulates the serum hEPO concentration by 7.2-fold over the vehicle control (mice). Cell permeable and low-toxic, ASP 7967 is orally bioavailable.

Technical Data for ASP 7967

M. Wt	464.44
Formula	C₂₀H₁₆F₂N₈.½C₄H₄O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1246958-42-8
PubChem ID	46931459
InChi Key	PQVNSJGSJVAEAC-WXXKFALUSA-N
Smiles	FC1=CC=C(C=C1)NC2=NC(NCC3=NC=CC=N3)=NC(NC4=CC=C(F)C=C4)=N2.OC(/C=C/C(O)=O)=O.FC5=CC=C(C=C5)NC6=NC(NCC7=NC=CC=N7)=NC(NC8=CC=C(F)C=C8)=N6

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ASP 7967

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.44	100

Preparing Stock Solutions for ASP 7967

The following data is based on the product molecular weight 464.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.15 mL	10.77 mL	21.53 mL
5 mM	0.43 mL	2.15 mL	4.31 mL
10 mM	0.22 mL	1.08 mL	2.15 mL
50 mM	0.04 mL	0.22 mL	0.43 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for ASP 7967

References are publications that support the biological activity of the product.

Kläge A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture. RNA Biology 2024 PMID: 38146121
Wang Integrating ligands into nucleic acid systems. ChemBioChem 2023 PMID: 37401635
Olenginski Flipping the script: understanding riboswitches from an alternative perspective. J.Biol.Chem. 2024 PMID: 38336293
Takahashi RNA-based controllers for engineering gene and cell therapies. Curr.Opin.Biotech. 2024 PMID: 38052131
Fukunaga Small-molecule aptamer for regulating RNA functions in mammalian cells and animals. J.Am.Chem.Soc. 2023 PMID: 36991533
Teixeira Synthetic gene circuits for regulation of next-generation cell-based therapeutics. Adv.Sci. 2024 PMID: 38126677
Salado Optimization of the pharmacokinetic properties of potent anti trypanosomal triazine derivatives. Eur.J.Med.Chem. 2018 PMID: 29604541