Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Name: Simple Plex Mouse/Rat Osteopontin/OPN Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-004049

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-004049

Simple Plex Mouse/Rat Osteopontin/OPN assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Serum (10 uL), Rat Serum (10 uL), Mouse EDTA Plasma (10 uL), Rat EDTA Plasma (10 uL), Mouse Heparin Plasma (10 uL), Mouse Urine (10 uL), Rat Urine (10 uL), Mouse Cerebrospinal Fluid (10 uL)
Sensitivity:	0.28 pg/mL
Assay Range:	1.2 - 4,580 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid, Mouse Cerebrospinal Fluid)
Simple Plex Mouse/Rat Osteopontin/OPN Cartridge Summary:	Simple Plex assay is for the detection of mouse and rat Osteopontin in serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse/rat Osteopontin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Osteopontin assay

Inter-Assay Precision (Precision between assays) Replicates of each Osteopontin control were tested in multiple assays performed by at least three technicians using two lots of reagents for Osteopontin

Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid, Mouse Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	19
Mean (pg/mL)	24.7	1229	24.2	1210
Standard Deviation	1.03	117	2.12	130
CV%	4.2	9.5	8.7	10.8

Recovery

Recovery for the mouse/rat Osteopontin assays was evaluated at three different spiked concentrations of Osteopontin protein.

Sample Type	Average % Recovery	Range %
Urine (n=4)	108	96-120

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Osteopontin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Rat Osteopontin Assay Linearity

Scientific Data Examples for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

	Simple Plex Mouse/Rat Osteopontin Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat Osteopontin Assay. Assay range is 1.20-4580 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Item	Value
Long Name	Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names	BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, Osteopontin, SPP-1, SPP1/CALPHA1 fusion, Spp1, Urinary stone protein, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), uropontin

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

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