Simple Plex Human VCAM-1/CD106 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000227
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human VCAM-1/CD106 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 9 |
| Mean (pg/mL) | 732 | 28,218 | 629 | 27,484 |
| Standard Deviation | 41.3 | 1686 | 66.6 | 1431 |
| CV% | 5.6 | 6.0 | 10.6 | 5.2 |
Recovery for Simple Plex Human VCAM-1/CD106 Cartridge
Recovery was not necessary as VCAM-1 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human VCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human VCAM-1/CD106 Cartridge
Human VCAM-1 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: VCAM-1/CD106
VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.
Long Name
Alternate Names
Gene Symbol
Additional VCAM-1/CD106 Products
Product Documents for Simple Plex Human VCAM-1/CD106 Cartridge
Product Specific Notices for Simple Plex Human VCAM-1/CD106 Cartridge
For research use only