Simple Plex Human PlGF Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000261
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human PlGF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 1 |
| n | 16 | 16 | 7 | 6 |
| Mean (pg/mL) | 34.9 | 1479 | 31.8 | 1614 |
| Standard Deviation | 1.56 | 73.6 | 1.89 | 178 |
| CV% | 4.5 | 5.0 | 6.0 | 11.0 |
Recovery for Simple Plex Human PlGF Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=3) | 91 | 87-93 |
| Heparin Plasma (n=3) | 86 | 78-91 |
| Serum (n=3) | 83 | 73-90 |
Linearity
Samples containing and/or spiked with high concentrations of human PIGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human PlGF Cartridge
Human PlGF Simple Plex Assay
Preparation and Storage
Shipping
Stability & Storage
Background: PlGF
PlGF (placenta growth factor) is secreted primarily by villous trophoblasts and decidual cells, with circulating levels increasing during pregnancy but attenuated in preeclampsia. PlGF signals through VEGF R1/Flt‑1 and Neuropilins, while VEGF binds both VEGF R1 and R2 but signals mainly through VEGF R2. PlGF reduces binding of VEGF to VEGF R1 and increases VEGF/VEGF R2‑mediated angiogenesis. It induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing and also contribute to inflammation in active sickle cell disease and atherosclerosis.
Long Name
Alternate Names
Gene Symbol
Additional PlGF Products
Product Documents for Simple Plex Human PlGF Cartridge
Product Specific Notices for Simple Plex Human PlGF Cartridge
For research use only