Simple Plex Human Nectin-2 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-006536
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Nectin-2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Nectin-2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Nectin-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Nectin-2.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 38 | 38 |
| Mean (pg/mL) | 29.7 | 1618 | 29.2 | 1417 |
| Standard Deviation | 2.47 | 131.9 | 2.79 | 105 |
| CV% | 8.3 | 8.2 | 9.6 | 7.4 |
Recovery for Simple Plex Human Nectin-2 Cartridge
Recovery was not necessary as human Nectin-2 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Nectin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Nectin-2 Cartridge
Simple Plex Human Nectin-2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Nectin-2 Assay. Assay range is 6.55-4000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Nectin-2/CD112
Nectins are type I transmembrane glycoproteins that are calcium-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs). Homology has led to their designation as poliovirus receptor-related (PRR) proteins, but nectins do not bind poliovirus. The extracellular domains of the nectin family form cis-homodimers (same cell), followed by homophilic or heterophilic trans-dimers (across cells) that organize intercellular junctions. Nectin-1, also known as CD111, PRR1 and herpes virus entry mediator C (HVEC) has a secreted splice variant. Nectin-1, Nectin-2 (CD112, PRR2, HVEB) and Nectin-3 (PRR3) are concentrated in adherens junctions and exist on neurons, endothelial cells, epithelial cells and fibroblasts. Nectin-4 is mainly expressed in the placenta; a soluble form is generated via the action of TACE/ADAM17.
Long Name
Alternate Names
Gene Symbol
Additional Nectin-2/CD112 Products
Product Documents for Simple Plex Human Nectin-2 Cartridge
Product Specific Notices for Simple Plex Human Nectin-2 Cartridge
For research use only