Simple Plex Human FAP Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000769
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human FAP Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FAP assay.
Inter-Assay Precision (Precision between assays) Replicates of each FAP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FAP.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 17 |
| Mean (pg/mL) | 227 | 10,581 | 227 | 11,364 |
| Standard Deviation | 5.27 | 731 | 19.9 | 621 |
| CV% | 2.3 | 6.9 | 8.8 | 5.5 |
Recovery for Simple Plex Human FAP Cartridge
Recovery was not necessary as human FAP shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human FAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human FAP Cartridge
Simple Plex Human FAP Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FAP Assay. Assay range is 10.5-40,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as seprase) is a transmembrane serine protease, and a soluble and enzymatically active form of FAP known as antiplasmin-cleaving enzyme (APCE) circulates in human plasma. FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV/CD26, which is specific for N-terminal Xaa-Pro sequences. FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion. In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity.
Alternate Names
Gene Symbol
Additional Fibroblast Activation Protein alpha/FAP Products
- All Products for Fibroblast Activation Protein alpha/FAP
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- Fibroblast Activation Protein alpha/FAP Lysates
- Fibroblast Activation Protein alpha/FAP Primary Antibodies
- Fibroblast Activation Protein alpha/FAP Proteins and Enzymes
- Fibroblast Activation Protein alpha/FAP Simple Plex
Product Documents for Simple Plex Human FAP Cartridge
Product Specific Notices for Simple Plex Human FAP Cartridge
For research use only