Simple Plex Human Endoglin/CD105 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000371
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Endoglin/CD105 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Endoglin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Endoglin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Endoglin.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 17 |
| Mean (pg/mL) | 951 | 45,292 | 1001 | 49,876 |
| Standard Deviation | 90.5 | 1760 | 74.9 | 4456 |
| CV% | 9.5 | 3.9 | 7.5 | 8.9 |
Recovery for Simple Plex Human Endoglin/CD105 Cartridge
Recovery was not necessary as human Endoglin shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Endoglin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Endoglin/CD105 Cartridge
Simple Plex Human Endoglin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Endoglin Assay. Assay range is 15.4-147,150 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Endoglin/CD105
Endoglin (CD105) is a transmembrane type III receptor for TGF-beta superfamily ligands and plays an important role in smooth muscle differentiation, angiogenesis, and neovascularization. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. Human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. Elevated levels of anti-angiogenic soluble Endoglin contribute to pathogenicity in preeclampsia. Endoglin associates with the receptors TGF-beta RII, Activin RIIA or RIIB, BMPR-IA/ALK-3, or BMPR-IB/ALK6 and enhance binding of Activin A, BMP-2, -7, -9, TGF-beta 1, or TGF-beta 3. Endoglin can either enhance or inhibit signaling through these receptor complexes.
Alternate Names
Gene Symbol
Additional Endoglin/CD105 Products
Product Documents for Simple Plex Human Endoglin/CD105 Cartridge
Product Specific Notices for Simple Plex Human Endoglin/CD105 Cartridge
For research use only