Simple Plex Human CX3CL1/Fractalkine Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000488
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CX3CL1/Fractalkine Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CX3CL1/Fractalkine assay.
Inter-Assay Precision (Precision between assays) Replicates of each CX3CL1/Fractalkine control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CX3CL1/Fractalkine.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 12 | 12 |
| Mean (pg/mL) | 504 | 26912 | 499 | 25992 |
| Standard Deviation | 23.1 | 1598 | 26.0 | 1434 |
| CV% | 4.6 | 5.9 | 5.2 | 5.5 |
Recovery for Simple Plex Human CX3CL1/Fractalkine Cartridge
Recovery for the human CX3CL1/Fractalkine assays was evaluated at three different spiked concentrations of CX3CL1/Fractalkine protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 110 | 102-127 |
| EDTA Plasma (n=4) | 101 | 93-112 |
| Heparin Plasma (n=4) | 99 | 93-113 |
| Serum (n=4) | 112 | 103-121 |
| Urine (n=6) | 92 | 77-103 |
Linearity
Samples containing and/or spiked with high concentrations of human CX3CL1/Fractalkine were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CX3CL1/Fractalkine Cartridge
Simple Plex Human CX3CL1/Fractalkine Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CX3CL1/Fractalkine Assay. Assay range is 56.8-86,710 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CX3CL1/Fractalkine
CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
Alternate Names
Gene Symbol
Additional CX3CL1/Fractalkine Products
Product Documents for Simple Plex Human CX3CL1/Fractalkine Cartridge
Product Specific Notices for Simple Plex Human CX3CL1/Fractalkine Cartridge
For research use only