Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000243
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 22 | 22 |
| Mean (pg/mL) | 5.54 | 241 | 5.48 | 242 |
| Standard Deviation | 0.434 | 15.9 | 0.266 | 26.7 |
| CV% | 7.8 | 6.6 | 4.8 | 11.0 |
Recovery for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 100 | 100-100 |
Linearity
Samples containing and/or spiked with high concentrations of human BAFF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge
Human BAFF/BLyS Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: BAFF/BLyS/TNFSF13B
BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
Long Name
Alternate Names
Gene Symbol
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge
Product Specific Notices for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge
For research use only