Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-014623
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TRANCE assay.
Inter-Assay Precision (Precision between assays) Replicates of each TRANCE control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TRANCE.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 24 | 24 |
| Mean (pg/mL) | 198 | 9796 | 253 | 12325 |
| Standard Deviation | 8.65 | 482 | 28.1 | 1286 |
| CV% | 4.4 | 4.9 | 11.1 | 10.4 |
Recovery for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay
Recovery for the human TRANCE assays was evaluated at three different spiked concentrations of TRANCE protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 112 | 107-117 |
| EDTA Plasma (n=2) | 98 | 81-116 |
| Heparin Plasma (n=2) | 114 | 109-120 |
| Serum (n=2) | 108 | 101-115 |
Linearity
Samples containing and/or spiked with high concentrations of human TRANCE were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TRANCE Assay. Assay range is 21.0-32,000 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: TRANCE/TNFSF11/RANK L
TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.
Long Name
Alternate Names
Gene Symbol
Additional TRANCE/TNFSF11/RANK L Products
Product Documents for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay
Product Specific Notices for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay
For research use only