Simple Plex Discovery Human FCRL5/FcRH5 Carridge New

Name: Simple Plex Discovery Human FCRL5/FcRH5 Carridge
Brand: ProteinSimple
SKU: SPCKB-PS-016663

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-016663

COA

Simple Plex Discovery Human FCRL5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	5.66 pg/mL
Assay Range:	13.1 - 20,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human FCRL5/FcRH5 Carridge Summary:	Simple Plex Discovery assay is for the detection of human Fc Receptor-Like 5 (FCRL5/FcRH5) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge with 3 Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human FCRL5/FcRH5.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FCRL5/FcRH5 assay

Inter-Assay Precision (Precision between assays) Replicates of each FCRL5/FcRH5 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FCRL5/FcRH5

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	28	28
Mean (pg/mL)	151	7583	141	6916
Standard Deviation	9.98	275	11	595
CV%	6.6	3.6	7.8	8.6

Recovery

Recovery for the human FCRL5/FcRH5 assays was evaluated at three different spiked concentrations of FCRL5/FcRH5 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	107	103-111

Linearity

Samples containing and/or spiked with high concentrations of FCRL5/FcRH5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

	Simple Plex Discovery Human FCRL5/FcRH5 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human FCRL5/FcRH5 Assay. Assay range is 13.1-20,000 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FCRL5

Fc receptor homologs, also known as IRTA or IFGP (IgSF, FcR, gp42) proteins, are transmembrane proteins with sequence homology to classical Fc receptors. FcRH have from three to nine Ig-like domains in their extracellular region and variable combinations of cytoplasmic immunotyrosine activation or inhibitory motifs (ITAM or ITIM). FcRH function as activating or inhibitory receptors on discrete B cell populations and are involved in lymphocyte development and transformation. No ligands for these proteins have been identified.

Item	Value
Long Name	Fc Receptor-like 5
Entrez Gene IDs	83416 (Human); 329693 (Mouse)
Alternate Names	BXMAS1, BXMAS1MGC119592, CD307, CD307e, CD307e antigen, DKFZp667E2019, DKFZp667F216, FCRH5MGC119593, FCRL5, FLJ00333, FLJ00397, Fc receptor homolog 5, Fc receptor-like 5, Fc receptor-like protein 5, FcR-like protein 5, FcRH5, IFGP5, IRTA2, IRTA2MGC119590, Immune receptor translocation-associated protein 2, PRO820, immunoglobulin superfamily receptor translocation associated 2 (IRTA2)