Non-Human Primate CCL2/MCP-1 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011281
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Non-Human Primate CCL2/MCP-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 18 |
| Mean (pg/mL) | 33.2 | 1746 | 32.3 | 1744 |
| Standard Deviation | 1.19 | 124 | 2.05 | 136 |
| CV% | 3.6 | 7.1 | 6.3 | 7.8 |
Recovery for Non-Human Primate CCL2/MCP-1 Cartridge
Recovery was not necessary as MCP-1 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of MCP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Non-Human Primate CCL2/MCP-1 Cartridge
Non-Human Primate CCL2/MCP-1 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL2/JE/MCP-1
CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
Alternate Names
Gene Symbol
Additional CCL2/JE/MCP-1 Products
Product Documents for Non-Human Primate CCL2/MCP-1 Cartridge
Product Specific Notices for Non-Human Primate CCL2/MCP-1 Cartridge
For research use only