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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Catalog # F0129 | R&D Systems, Inc. a Bio-Techne Brand
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F0129

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 344701

Product Summary for Rat Anti-Mouse IgG2A PE-conjugated Antibody

Immunogen

Cocktail of mouse IgG1 and IgG2A

Specificity

The specificity of this antibody was confirmed by direct ELISA on mouse IgG2A monoclonal antibodies, and it does not cross-react with IgG1, IgG2B, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2A isotype, and does not detect primary antibodies of mouse IgG1, IgG2B, or IgG3 class.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Rat Anti-Mouse IgG2A PE-conjugated Antibody

Detection of Mouse IgG2APrimary Antibody by Flow Cytometry.

Detection of Mouse IgG2APrimary Antibody by Flow Cytometry.

Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by PE-conjugated Rat Anti-Mouse IgG2Aisotype-specific secondary antibody (Catalog # F0129). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat Anti-Mouse IgG2A PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse IgG2A Primary Antibody
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using F0129 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Alternate Names

IgG2a

Product Documents for Rat Anti-Mouse IgG2A PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Anti-Mouse IgG2A PE-conjugated Antibody

For research use only

Citations for Rat Anti-Mouse IgG2A PE-conjugated Antibody (3)

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