Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all Kappa Light Chain Secondary Antibodies »

Product Specifications

Immunogen

Human Kappa Light Chain

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No cross-reactivity was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Human Kappa Light Chain Secondary Antibody

Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463]

Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463]

Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.

Applications for Goat anti-Human Kappa Light Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200- 1:2000

Immunohistochemistry

1:200- 1:2000

Immunohistochemistry-Paraffin

1:200- 1:2000

Western Blot

1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Reviewed Applications

Read 1 review rated 5 using NB7463 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Goat anti-Human Kappa Light Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human Kappa Light Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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