Rabbit anti-Human IgM Heavy Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72778
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rabbit anti-Human IgM Fc5u Secondary Antibody was produced by repeated immunization with Human IgM Fc5u fragment in rabbit.
Specificity
Specific to Fc5u portion of IgM. No reaction was observed against Human IgG or Human IgA.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgM and Human Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Applications for Rabbit anti-Human IgM Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:50000
Immunohistochemistry
1:500 - 1:3000
Western Blot
1:500 - 1:3000
Application Notes
This product has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Rabbit anti-Human IgM Heavy Chain Secondary Antibody
Product Specific Notices for Rabbit anti-Human IgM Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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