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Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer]

Novus Biologicals, part of Bio-Techne | Catalog # MP-5401-NB

Novus Biologicals, part of Bio-Techne
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MP-5401-NB-15ml
MP-5401-NB-50ml

Key Product Details

Species Reactivity

Rabbit

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, In-situ Hybridization

Label

AP Polymer

Antibody Source

Polyclonal Horse IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Rabbit IgG

Clonality

Polyclonal

Host

Horse

Isotype

IgG

Description

Features:
  • High sensitivity
  • Low background
  • One-step detection
  • Micropolymer technology
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Scientific Data Images for Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer]

    Immunocytochemistry/Immunofluorescence: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue). Visualized under fluorescence. Broad excitation with peak at 500nm and broad emission with peak at 680nm).
    Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Colon Cancer: Anti-Ki67 (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue); Anti-Cox2 (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, ImmPACT Vector Red Substrate (red).
    Immunohistochemistry-Paraffin: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, BCIP/NBT Substrate (indigo).

    Applications for Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer]

    Application
    Recommended Usage

    ELISA

    Optimal dilutions of this antibody should be experimentally determined.

    Immunocytochemistry/ Immunofluorescence

    Optimal dilutions of this antibody should be experimentally determined.

    Immunohistochemistry

    Optimal dilutions of this antibody should be experimentally determined.

    In-situ Hybridization

    Optimal dilutions of this antibody should be experimentally determined.
    Application Notes
    .

    Formulation, Preparation, and Storage

    Purification

    Affinity purified

    Preservative

    No Preservative

    Concentration

    Please see the vial label for concentration. If unlisted please contact technical services.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Stability & Storage

    Store at 4C. Do not freeze.

    Background: IgG ImmPRESS(TM)

    The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications.

    Additional IgG ImmPRESS(TM) Products

    Product Documents for Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer]

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Product Specific Notices for Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer]

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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