Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Goat anti-Mouse IgG (H+L) Secondary Antibody was produced by repeated immunization with Mouse IgG whole molecule in goat.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.

Store at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Mouse IgG (H+L) Secondary Antibody

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702]

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702]

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702] - Lane 1: Mouse IgG whole molecule. Lane 2: Mouse IgG F(c) Fragment. Lane 3: Mouse IgG F(ab) Fragment. Lane 4: Mouse IgM Kappa. Lane 5: Mouse Serum. Load: 50ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG 1:40,000 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702]

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702]

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4C. Secondary antibody: DyLight 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.
Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72702] - Dot Blot of Goat anti-Mouse IgG (H+L) Secondary Antibody [Texas Red]. Lane 1: 100 ng: Lane 2: 33.3 ng. Lane 3: 11.1 ng. Lane 4: 3.7 ng. Lane 5: 1.23 ng. Blocked for 30 min at room temperature. Image from the Texas Red version of this antibody.

Applications for Goat anti-Mouse IgG (H+L) Secondary Antibody

Application
Recommended Usage

ELISA

1:25000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:3000 - 1:15000
Application Notes
This product is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Mouse IgG (H+L) Secondary Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Goat anti-Mouse IgG (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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