Mouse Pure-Blot anti-Sheep IgG (H+L) Secondary Antibody (eB270) [HRP]

This secondary antibody has been tested in western blot and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Sheep IgG Pure-Blot is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep Pure-Blot secondaries, but not with the rabbit Pure-Blot secondary. Use of protein A or G beads with the rabbit Pure-Blot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.