Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6252
![SDS-PAGE: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed) [NB120-6252]](https://resources.bio-techne.com/images/products/Goat-F-ab-2-anti-Rat-IgG-Fc-Secondary-Antibody-Pre-adsorbed-SDS-Page-NB120-6252-img0002.jpg "SDS-PAGE: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed) [NB120-6252]")
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Rat F(c) fragment in goat.
Specificity
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed)
SDS-PAGE: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed) [NB120-6252]
SDS-Page: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed) [NB120-6252] - SDS-PAGE results of Goat F(ab')2 anti-Rat IgG Fc Secondary antibody (Pre-adsorbed).Lane 1: reduced Goat F(ab')2 anti-Rat.Lane 2: Opal Prestained LadderLane 3: non-reduced Goat F(ab')2 anti-Rat.Load: 1.0ug.4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.Applications for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunohistochemistry
1:1000 - 1:3000
Western Blot
1:2000 - 1:5000
Application Notes
This product has been tested by SDS=PAGE and is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Pre-adsorbed
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed)
Product Specific Notices for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...