Rabbit anti-Bovine IgA/Secretory Component Secondary Antibody

Bovine

ELISA, Immunodiffusion, Immunoelectrophoresis

Unconjugated

Polyclonal Rabbit

LYOPH mg/ml

Highly purified IgA/Secretory Component isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

The antiSerum does not cross-react with any other component of the Bovine Ig system. Interspecies cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal bovine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal bovine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA.

Polyclonal

Rabbit

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Whole Antiserum

Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Delipidated, heat inactivated lyophilized whole antiserum

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.