Recombinant Mouse VEGF 164 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76335
Key Product Details
Source
Yeast
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains corresponding to VEGF 164. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from approximately 40 kDa in SDS-PAGE under non-reducing conditions Source: Yeast
Amino Acid Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.01 EU/ug of VEGF 164 as determined by LAL method.
Predicted Molecular Mass
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-5.0 ng/ml.
Application Notes
As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from approximately 40 kDa in SDS-PAGE under non-reducing conditions.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-76335
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: VEGF 164
Gene Symbol
VEGFA
Additional VEGF 164 Products
Product Documents for Recombinant Mouse VEGF 164 Protein
Product Specific Notices for Recombinant Mouse VEGF 164 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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