Recombinant E. coli Uridine Phosphorylase 1/UPP1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46053

Novus Biologicals, part of Bio-Techne
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NBP1-46053-0.1mg
NBP1-46053-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Uridine Phosphorylase 1/UPP1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-253 of E.coli Uridine Phosphorylase 1/UPP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Uridine Phosphorylase 1/UPP1 His Protein

SDS-PAGE: Recombinant E. coli Uridine Phosphorylase 1/UPP1 His Protein [NBP1-46053]

SDS-PAGE: Recombinant E. coli Uridine Phosphorylase 1/UPP1 His Protein [NBP1-46053]

SDS-Page: Recombinant E. coli Uridine Phosphorylase 1/UPP1 Protein [NBP1-46053] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-46053
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 50 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Uridine Phosphorylase 1/UPP1

Uridine phosphorypase(UDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, UrdPase, UPH, and UPase. Recombinant E.coli UDP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

UDPRASE, UP, UPASE 1, UPP, UrdPase 1

Gene Symbol

UPP1

Additional Uridine Phosphorylase 1/UPP1 Products

Product Documents for Recombinant E. coli Uridine Phosphorylase 1/UPP1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli Uridine Phosphorylase 1/UPP1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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