Recombinant T. maritima alpha-L-Fucosidase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6556-GH

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6556-GH-020
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Key Product Details

  • R&D Systems E. coli-derived Recombinant T. maritima alpha-L-Fucosidase Protein (6556-GH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

NP_228118

Conjugate

Unconjugated

Applications

Enzyme Activity

View all alpha-L-Fucosidase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived t. maritima alpha-L-Fucosidase protein
Ile2-Glu449, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

53 kDa

SDS-PAGE

43-50 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic substrate 4-methylumbelliferyl-alpha -L-fucopyranoside.
The specific activity is >1,600 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 6556-GH in the following applications:

Formulation, Preparation and Storage

6556-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha-L-Fucosidase

Fucosylated glycoconjugates play numerous roles in biological events, including development and apoptosis (1, 2), and are involved in the pathology of inflammation, cancer, and cystic fibrosis (3, 4). Fucosidases are generally used for studying fucosylated glycans. With a Kcat/Km value of 160,000 M-1s-1, the alpha-L-fucosidases of Thermotoga maritima efficiently hydrolyzes 4-nitrophenyl fucoside (5). The enzyme is the closest bacterial relative of mammalian fucosidase with 38% identity to its human homologue. It is thought to remove alpha‑1,2‑ and alpha-1,4-linked fucosyl side chains from algal fucoidan (5), while the activity on alpha-1,3- and alpha‑1,6‑ linked fucose has not been tested. The enzyme assembles as a hexamer and displays a two-domain fold, with Asp224 and Glu266 being critical for enzyme activity (6).

References

  1. Hiraishi, K. et al. (1993) Glycobiology 3:381.
  2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  3. Shah, M. et al. (2008) cancer 113:338.
  4. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
  5. Tarling, C.A. et al. (2003) J. Biol. Chem. 278:47394.
  6. Sulzenbacher, G. et al. (2004) J. Biol. Chem. 279:13119.

Alternate Names

alphaLFucosidase

Entrez Gene IDs

897238 (T. maritima)

Gene Symbol

TM_RS01570

UniProt

NP_228118

Additional alpha-L-Fucosidase Products

Product Documents for Recombinant T. maritima alpha-L-Fucosidase Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant T. maritima alpha-L-Fucosidase Protein, CF

For research use only

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