Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10630-CV

R&D Systems, part of Bio-Techne
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10630-CV-100
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Key Product Details

Source

E. coli

Accession #

YP_009725306.1

Conjugate

Unconjugated

View all NSP10 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived sars-cov-2 NSP10 protein
Ala1-Gln139
with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala1

Predicted Molecular Mass

16 kDa

SDS-PAGE

16 kDa, under reducing conditions

Reviewed Applications

Read 1 review rated 5 using 10630-CV in the following applications:

Scientific Data Images for Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF

Recombinant SARS-CoV-2 NSP10 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV-2 NSP10 His-tag (Catalog # 10630-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 16 kDa under reducing conditions.

Formulation, Preparation and Storage

10630-CV
Formulation Supplied as a 0.2 μm filtered solution in HEPES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: NSP10

Non-structural protein 10 (NSP10), is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP10 is a small 139 amino acid protein that forms independent dodecameric structures composed of four identical trimers where each monomer is capable of binding two zinc ions (2, 3) that are coordinated uniquely and has exposed basic residues on the protein's surface consequently allowing RNA-binding (3). The NSP10 sequence is conserved across coronaviruses (4) and is thought to serve as an important multifunctional cofactor in viral replication. NSP10 was shown to interact with other NSP proteins including NSP14 and NSP16 (5). The N-terminus of NSP10 has been shown to interact with NSP14 and proposed to stabilize and regulate the 3'-5' exoribonuclease domain activity while not directly impacting the methyltransferase activity of NSP14 (6,7). A similar region of NSP10 interacts with NSP16; this interaction is required for stabilization of NSP16 and stimulation of its 2'-O-methyltransferase activity (4,7). Consequently, NSP10 is critically involved in the formation of functional viral mRNA capping machinery in coronaviruses and a potential target to reduce viral replication and pathogenesis (8,9). 

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59. 
  2. Matthes, N. et al. (2006) FEBS Lett. 580:4143.
  3. Su, D. et al. (2006) J. Virol. 80:7902.
  4. Rosas-Lemus, M. et al. (2020) bioRxiv. In press.
  5. Pan, J. et al. (2008) PLoS One 3:e3299.
  6. Bouvet, M. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:9372.
  7. Bouvet, M. et al. (2014) J. Biol. Chem. 289:25783.
  8. Ma, Y. et al. (2015) Proc. Natl. Acad. Sci. 112:9436.
  9. Wang, Y. et al. (2015) J. Virol. 89:8416.

Long Name

Non-Structural Protein 10

UniProt

YP_009725306.1

Additional NSP10 Products

Product Documents for Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF

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Product Specific Notices for Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF

For research use only

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