Recombinant S. plicatus Endo H Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5224-GHB

R&D Systems, part of Bio-Techne
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5224-GHB-050
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Key Product Details

Source

E. coli

Accession #

P04067

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Endo-beta-N-acetylglucosaminidase H/Endo H Proteins and Enzymes »

Product Specifications

Source

E. coli-derived s. plicatus Endo-beta-N-acetylglucosaminidase H/Endo H protein
Val47-Pro313 with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

MHHHHHHVKQ

Predicted Molecular Mass

30 kDa

SDS-PAGE

29 kDa, reducing conditions

Activity

Measured by its ability to deglycosylate ribonuclease B under native conditions.
The DC50 is <5 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 μg of RNase B in 30 minutes at
37 °C. . Use of Recombinant S. plicatus Endo-beta -N-acetylglucosaminidase H/Endo H in the deglycoslyation of other substrates may require alternative conditions for optimal performance.

Formulation, Preparation and Storage

5224-GHB
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Endo-beta-N-acetylglucosaminidase H/Endo H

N-glycans are commonly found on various glycoproteins. While peptide N-glycosidase from Flavobacterium meningosepticum (PNGase F) is widely used to release virtually all types of N-glycans under denaturing conditions, some bacterial endo-beta -N-acetylglucosaminidases, including Endo H, can be used under native conditions to specifically release particular types of N-glycans (1, 2). Because these glycosidases hydrolyze the chitobiose core of an N-glycan on a glycoprotein, the released glycan product will contain one GlcNAc residue at its reducing end with the other GlcNAc residue remaining attached to the glycoprotein. Endo H specifically releases oligomannose and hybrid but not complex type N-glycans from glycoproteins (3-5). It also remains active on core fucosylated N-glycans but not sulfated glycans (5).

References

  1. Maley, F. et al. (1989) Anal. Biochem. 180:195.
  2. Tarentino, A.L. et al. (1985) Biochemistry 24:4665.
  3. Hsieh, P. et al. (1983) J. Biol. Chem. 258:2555.
  4. Tarentino, A.L. et al. (1992) J. Biol. Chem. 267:3868.
  5. Trimble, R.B. and Tarentino, A.L. (1991) J. Biol. Chem. 266:1646.

Alternate Names

EndobetaNacetylglucosaminidase H

UniProt

P04067

Additional Endo-beta-N-acetylglucosaminidase H/Endo H Products

Product Documents for Recombinant S. plicatus Endo H Protein, CF

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Product Specific Notices for Recombinant S. plicatus Endo H Protein, CF

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