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Recombinant Rat IL-2 Protein

Catalog # 502-RL | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
502-RL-010/CF
502-RL-050/CF

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Availability
Size / Price
Qty
With Carrier
502-RL-010
502-RL-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived rat IL-2 protein
Ala21-Gln155, with and without an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21 & Met

Predicted Molecular Mass

16 kDa

Activity

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.
The ED50 for this effect is 0.04-0.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 502-RL
Formulation Supplied as a 0.2 μm filtered solution in Ammonium Acetate and Glycerol with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Carrier Free: 502-RL/CF
Formulation Supplied as a 0.2 μm filtered solution in Ammonium Acetate and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated four alpha-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1‑3). Mature rat IL-2 shares 66% and 73% amino acid sequence identity with human and mouse IL-2, respectively. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (4 - 6). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain  gammac/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gammac. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (7). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (8‑10). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (11, 12).

References

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. McKnight, A. et al. (1989) Immunogenetics 30:145.
  4. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  5. Wang, X. et al. (2005) Science 310:1159.
  6. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  7. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  8. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  9. Laurence, A. et al. (2007) Immunity 26:371.
  10. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  11. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  12. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

Product Documents for Recombinant Rat IL-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat IL-2 Protein

For research use only

Citations for Recombinant Rat IL-2 Protein (10)

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  • Recombinant Rat IL-2 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/08/2017
    The recombinant rat IL-2 was used to build an ELISA standard for the quantification of rat IL-2 in serum samples. The ELISA was built using AF502 and BAF502 polyclonal antibodies.
    Recombinant Rat IL-2 Protein 502-RL

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