Recombinant P. chrysogenum APS kinase/APSK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7176-SK

R&D Systems, part of Bio-Techne
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7176-SK-020
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Key Product Details

  • R&D Systems E. coli-derived Recombinant P. chrysogenum APS kinase/APSK Protein (7176-SK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

Q12657

Conjugate

Unconjugated

Applications

Enzyme Activity

View all APS Kinase/APSK Proteins and Enzymes »

Product Specifications

Source

E. coli-derived p. chrysogenum APS Kinase/APSK protein
Met1-Glu211, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

25 kDa

SDS-PAGE

29-30 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate adenosine 5'-phosphosulfate (APS).
The specific activity is >100 pmol/min/μg, as measured under the described conditions, and is subject to known limitations imposed by a factor of coupling rate (8).

Formulation, Preparation and Storage

7176-SK
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: APS Kinase/APSK

Sulfur metabolism is essential to all organisms. Inorganic sulfate is sequentially activated by ATP sulfurylase and adenosine 5′‑phosphosulfate kinase (APSK). ATP sulfurylase converts ATP to adenosine‑5'‑phosphosulfate (APS) (1). APSK subsequently phosphorylates APS at the 3’ site to generate 3'‑phosphoadenosine‑5'‑phosphosulfate (PAPS) (2). PAPS is the sulfur donor required for all sulfotransferase reactions in humans and is also the sulfur source for synthesis of cysteine, methionine and glutathione (3). The P. chrysogenum APSK is a homodimer that undergoes a temperature‑dependent reversible dissociation into inactive monomers (4). The kinetic mechanism of this enzyme is strictly ordered with ATP bound before APS and PAPS dissociating before MgADP. APS is reported as a potent substrate inhibitor with the formation of a dead‑end E·MgADP·APS ternary complex as the cause of the substrate inhibition (5). The recombinant P. chrysogenum APSK is required for in vitro PAPS synthesis (6, 7) and the enzymatic activity was measured using a phosphatase‑coupled method (8).

References

  1. Robbins, P. W. and Lipmann, F. (1958) J. Biol. Chem. 233:686.
  2. MacRae, I.J. et al. (1998) J. Biol. Chem. 273:28583.
  3. Strott, C.A. (2002) Endocr. Rev 23:703.
  4. Renosto, F. et al. (1985) J. Biol. Chem. 260:1535.
  5. Renosto, F. et al. (1991) Arch. Biochem. Biophys. 284:30.
  6. Wu, Z.L. et al. (2002) The FASEB J. 16:539.
  7. Lin, C.H. et al. (1995) J. Am.Chem. Soc. 117:8031.
  8. Wu, Z.L. (2011) PLoS ONE 6(8): e23172.

Long Name

Adenosine-5'-phosphosulfate 3'-phosphotransferase

Alternate Names

APSK

UniProt

Q12657

Additional APS Kinase/APSK Products

Product Documents for Recombinant P. chrysogenum APS kinase/APSK Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant P. chrysogenum APS kinase/APSK Protein, CF

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