Recombinant Mouse TL1A/TNFSF15 Protein

Name: Recombinant Mouse TL1A/TNFSF15 Protein
Brand: R&D Systems
SKU: 1896-TL
Rating: 5 (1 reviews)

Catalog # 1896-TL | R&D Systems, Inc. a Bio-Techne Brand

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Citations (16)

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Key Product Details

Source:	E. coli
Accession #:	AAM77367
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse TL1A/TNFSF15 Protein

Technical Data
Citations (16) / Reviews (1)

Product Specifications for Recombinant Mouse TL1A/TNFSF15 Protein

Source	E. coli-derived mouse TL1A/TNFSF15 protein Ile76-Leu252, with an N-terminal Met
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	20 kDa
SDS-PAGE	20 kDa
Activity	Measured by its ability to induce apoptosis of TF‑1 human erythroleukemic cells. Migone, T. et al. (2002) Immunity 16:479. The ED₅₀ for this effect is 15-75 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1896-TL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1896-TL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). Mouse TL1A is a 252 amino acid residues (aa) protein consisting of a 35 aa N-terminal cytoplasmic domain, a 24 aa transmembrane region and 193 aa C-terminal extracellular domain (1). TL1A is expressed predominantly in endothelial cells and its expression is stimulated by TNF-a and IL-1a (1). Non-endothelial cells of the gut mucosa, including lamina propria lymphocytes and tissue macrophages, also express TL1A and at higher levels in chronic inflammatory bowel disorders (2). TL1A binds with high affinity to death receptor 3 (DR3), which is now designated TNF receptor superfamily member 25 (TNFRSF25). DR3 was formerly designated TNFRSF12 when it was thought to be the receptor for TWEAK/TNFSF12. Depending on the cell type, DR3-TL1A interactions have different effects. Ligation of DR3 on activated T cells by TL1A provides a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflamatory cytokines (1). The promotion of survival of activated T cells by TL1a results from the activation of the transcription factor NF-kappa-B. In a tumor erythroleukemic cell line, TF-1, DR3-TL1A signaling increases production of the NF-kB-dependent antiapoptotic protein c-IAP2, promoting survival in these cells (3). In HUVEC cells, which express both DR3 and TL1A, ligation of DR3 by TL1A regulates cell apoptosis (4). These effects of TL1A are blocked by the secreted, soluble decoy receptor 3 (DcR3), also known as TR6 and TNFRSF6B, which compete with DR3 for binding to TL1A (1-4). Consistent with the observed in vitro activities, TL1A promotes ex vivo splenocyte expansion and enhances in vivo graft-versus-host-response. The level of TL1A in cells of gut mucosa, in patients with bowel inflammatory disorders, correlates with the severity of inflammation, and TL1A may play a role in a Th1-mediate pathological conditions such as Crohn’s disease (2). Mouse and human TL1A share 73% homology in their C-terminal domains.

Item	Value
References	Migone, T.S. et al. (2002) Immunity 16:479. Bamias, G. et al. (2003) J. Immunol. 171:4868. Wen, L. et al. (2003) J. Biol. Chem. 278:39251. Yang, C.-R. et al. (2004) Cancer Res. 64:1122.
Long Name	TNF-like 1
Entrez Gene IDs	9966 (Human); 326623 (Mouse)
Alternate Names	MGC129934, MGC129935, TL1A, TL1Vascular endothelial cell growth inhibitor, TNF superfamily ligand TL1A, TNFSF15, VEGI, VEGI192A, VEGITNF ligand-related molecule 1, tumor necrosis factor (ligand) superfamily, member 15, tumor necrosis factor ligand superfamily member 15, vascular endothelial growth inhibitor-192A

Citations for Recombinant Mouse TL1A/TNFSF15 Protein (16)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TL1A/TNFSF15 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

N Jacob et al. (2020-10-23), Direct signaling of TL1A-DR3 on fibroblasts induces intestinal fibrosis in vivo Sci Rep, 2020-110(1):18189.
PMID: 33097818
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Jacob et al. (2018-07-09), Inflammation-independent TL1A-mediated intestinal fibrosis is dependent on the gut microbiome Mucosal Immunol, 2018-00(0).
PMID: 29988118
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA (Standard)

JR Ferdinand et al. (2018-01-15), Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells J. Immunol., 2018-00(0).
PMID: 29335258
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016-08-05), Dectin-1-activated dendritic cells trigger potent antitumour immunity through the induction of Th9 cells Nat Commun, 2016-07(0):12368.
PMID: 27492902
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Li-Guo Jia (2016-05-27), A Novel Role for TL1A/DR3 in Protection against Intestinal Injury and Infection J Immunol, 2016-0197(1):377-86.
PMID: 27233964
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Holmkvist et al. (2016-05-06), IL-18R?-deficient CD4(+) T cells induce intestinal inflammation in the CD45RB(hi) transfer model of colitis despite impaired innate responsiveness Eur J Immunol, 2016-00(0).
PMID: 27062602
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Gomez-Rodriguez J et al. (2016-03-03), Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4. Nat Commun, 2016-07(0):10857.
PMID: 26936133
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Longman R et al. (2014-07-14), CX(3)CR1(+) mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22. J Exp Med, 2014-0211(8):1571-83.
PMID: 25024136
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

Yu et al. (2013-11-13), TNF superfamily member TL1A elicits type 2 innate lymphoid cells at mucosal barriers. Mucosal Immunol, 2013-17(3):730-40.
PMID: 24220298
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wang X et al. (2013-10-18), TNF-like ligand 1A (TL1A) gene knockout leads to ameliorated collagen-induced arthritis in mice: implication of TL1A in humoral immune responses. J Immunol, 2013-1191(11):5420-9.
PMID: 24140642
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2012-06-04), Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-0109(25):9971-6.
PMID: 22665806
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Twohig J et al. (2012-05-16), The death receptor 3/TL1A pathway is essential for efficient development of antiviral CD4(+) and CD8(+) T-cell immunity. FASEB J, 2012-026(8):3575-86.
PMID: 22593543
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jones GW et al. (2010-09-08), Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J., 2010-025(1):409-19.
PMID: 20826539
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kayamuro H et al. (2009-05-04), TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009-0384(3):296-300.
PMID: 19406102
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mueller AM et al. (2009-03-06), The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid. J. Neuroimmunol., 2009-0209(1):57-64.
PMID: 19269042
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kim MY et al. (2008-01-16), Heterogeneity of lymphoid tissue inducer cell populations present in embryonic and adult mouse lymphoid tissues. Immunology, 2008-0124(2):166-74.
PMID: 18205791

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Recombinant Mouse TL1A/TNFSF15 Protein

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Reason for Rating: Recombinant Mouse TL1A is bound by patented anti-TL1a antibodies via direct ELISA.

Verified Customer | Posted 01/03/2020

Recombinant Mouse TL1A/TNFSF15 Protein

Catalog # 1896-TL | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Mouse TL1A/TNFSF15 Protein

Formulation, Preparation and Storage

Carrier Free

1896-TL

Carrier

1896-TL/CF

Carrier Free

Reconstitution Calculator

Background: TL1A/TNFSF15

Citations for Recombinant Mouse TL1A/TNFSF15 Protein (16)

Customer Reviews for Recombinant Mouse TL1A/TNFSF15 Protein (1)

Customer Images

Recombinant Mouse TL1A/TNFSF15 Protein

Product Documents for Recombinant Mouse TL1A/TNFSF15 Protein

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