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Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF

Catalog # 10207-IF | R&D Systems, Inc. a Bio-Techne Brand
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10207-IF-010
10207-IF-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 6/IFNA6 protein
Cys24-Glu189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

19-22 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is <100 pg/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF

Recombinant Mouse IFN-alpha 6/IFNA6 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 6/IFNA6 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 6/IFNA6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-22 kDa.
Recombinant Mouse IFN-alpha 6/IFNA6 Protein Bioactivity

Recombinant Mouse IFN-alpha 6/IFNA6 Protein Bioactivity

Recombinant Mouse IFN-alpha 6/IFN6 (Catalog # 10207-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is

Formulation, Preparation and Storage

10207-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 6/IFNA6

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). Mature mouse IFNA6 consists of 166 aa and shares 60% aa identity with human IFNA6. The type I IFNs binds to the interferon alpha receptor (IFNAR) which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA6 displaying the superior efficacy controlling influenza virus infection and disease (6). Treatment with IFNA6 DNA 2 weeks post‐MCMV infection proved effective at inhibiting the development of chronic autoimmune myocarditis. IFNA6 is also able to reduce chronic cardiac inflammation. These findings suggest that immunomodulation of both antiviral and autoimmune responses by IFN DNA immunization may be an avenue for improved viral immunotherapy (7, 8).

References

  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. Fung, K.Y. et al. (2013) Science 339:1088.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  7. Bartlett, E.J. et al. (2002) Immunol. Cell Biol. 5:425.
  8. Bartlett, E.J. et al. (2003) Biol. Proced. 5:43.

Long Name

Interferon alpha 6

Alternate Names

IFN-alpha K, IFNA6, IFNalpha K

Entrez Gene IDs

3443 (Human); 15969 (Mouse)

Gene Symbol

IFNA6

UniProt

Product Documents for Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF

For research use only

Reconstitution Calculator

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