Recombinant Mouse HAI-2B Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1107-PI
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse HAI-2B Protein (1107-PI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse HAI-2B protein
Arg30-Lys140, with a C-terminal 10-His tag
Arg30-Lys140, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg30
Predicted Molecular Mass
14 kDa
SDS-PAGE
23 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <2 nM, as measured under the described conditions.
The IC50 value is <2 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1107-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HAI-2B
Three alternatively spliced forms of HAI-2 have been found in mouse tissues (1). HAI-2A, the full-length molecule, consists of two Kunitz domains and a C-terminal transmembrane domain. HAI-2A is a major form expressed in human tissues, but a minor form in mouse tissues. HAI-2B, with residues 37‑93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin (2).
References
- Itoh, H. et al. (1999) Biochem. Biophys. Res. Comm. 255:740.
- Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
Long Name
HGF Activator Inhibitor Type 2B
Alternate Names
HAI2B
Entrez Gene IDs
20733 (Mouse)
Gene Symbol
SPINT2
UniProt
Additional HAI-2B Products
Product Documents for Recombinant Mouse HAI-2B Protein, CF
Product Specific Notices for Recombinant Mouse HAI-2B Protein, CF
For research use only
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