Recombinant Mouse Dkk-1 (Histidine-tagged) Protein
Catalog # 1765-DK | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Ser30-His272, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Recombinant Mouse Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 30-180 ng/mL.
Scientific Data Images for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein
Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Bioactivity
Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is 30-180 ng/mL (orange circles).Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1765-DK
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 1765-DK/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Dkk-1
References
- Glinka, A. et al. (1998) Nature 391:357.
- Niehrs, C. (2006) Oncogene 25:7469.
- Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
- Mao, B. et al. (2001) Nature 411:321.
- Mao, B. et al. (2002) Nature 417:664.
- Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
- Adamska, M. et al. (2004) Dev. Biol. 272:134.
- Li, J. et al. (2006) Bone 36:754.
- Verani, R. et al. (2006) J. Neurochem. 101:242.
- Pinzone, J.J. et al. (2009) Blood 113:517.
- Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
- Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein
Product Specific Notices for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein
For research use only
Citations for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein (8)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein include:
-
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayA Yachie-Kin et al. (2018-01-29), Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions Mol. Syst. Biol., 2018-014(1):e7952.
PMID: 29378814 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayTrowbridge JJ et al. (2010-08-01), Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells, 2010-028(8):1379-89.
PMID: 20521329 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayHansson M et al. (2009-04-07), A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev. Biol., 2009-0330(2):286-304.
PMID: 19358838 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayNakanishi M et al. (2008-09-22), Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium. FASEB J., 2008-023(1):114-22.
PMID: 18809738 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoChen Y et al. (2007-09-23), Very low density lipoprotein receptor, a negative regulator of the wnt signaling pathway and choroidal neovascularization. J. Biol. Chem., 2007-0282(47):34420-8.
PMID: 17890782 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayKwon C et al. (2007-06-18), Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors. Proc. Natl. Acad. Sci. U.S.A., 2007-0104(26):10894-9.
PMID: 17576928 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayWawrzak D et al. (2007-04-19), Wnt3a binds to several sFRPs in the nanomolar range. Biochem. Biophys. Res. Commun., 2007-0357(4):1119-23.
PMID: 17462603 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayLindsley RC et al. (2006-08-30), Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006-0133(19):3787-96.
PMID: 16943279
There are no citations that match your criteria.
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