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Recombinant Mouse Decorin Protein, CF

Catalog # 1060-DE | R&D Systems, Inc. a Bio-Techne Brand
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1060-DE-100

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Decorin protein
Gly17-Lys354 & Asp31-Lys354, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly17 & Asp31

Predicted Molecular Mass

39 kDa

SDS-PAGE

40-45 kDa, reducing conditions

Activity

Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626.
5 µg/mL of Recombinant Mouse Decorin can significantly delay the rate of collagen fibrillogenesis.

Formulation, Preparation and Storage

1060-DE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 ‑ 12 tandem leucine-rich repeats (LRR) (1). The mouse Decorin cDNA encodes a 354 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature mouse Decorin contains twelve tandem LRR and shares 80% and 87% aa sequence identity with human and rat Decorin, respectively. Decorin is an N-glycosylated protein that also carries a variably-sized hybrid chondroitin/dermatan sulfate chain at Ser34 (2, 3). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (4). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF-2, GDF-8/Myostatin, TGF-beta, and WISP-1 (3, 5 ‑ 8). It also binds and activates EGF R, ErbB4, and IGF-I R (9 ‑ 11). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (12 ‑ 15). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (16).

References

  1. Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305.
  2. Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270.
  3. Zamfir, A. et al. (2003) Glycobiology 13:733.
  4. Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823.
  5. Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887.
  6. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
  7. Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  8. Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599.
  9. Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468.
  10. Santra, M. et al. (2000) J. Biol. Chem. 275:35153.
  11. Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767.
  12. Olguin, H.C. et al. (2003) Dev. Biol. 259:209.
  13. Schonherr, E. et al. (2004) J. Vasc. Res. 41:499.
  14. Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408.
  15. Reed, C.C. et al. (2005) Oncogene 24:1104.
  16. Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.

Alternate Names

DCN, DSPG2, PG-II, PGS2, SLRR1B

Entrez Gene IDs

1634 (Human); 13179 (Mouse)

Gene Symbol

DCN

UniProt

Product Documents for Recombinant Mouse Decorin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Decorin Protein, CF

For research use only

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