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Recombinant Mouse CD200R1 His-tag Protein, CF

Catalog # 10054-CD | R&D Systems, Inc. a Bio-Techne Brand
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10054-CD-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD200 R1 protein
Thr26 - Pro238, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr26

Predicted Molecular Mass

24 kDa

SDS-PAGE

50-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant  Mouse CD200 R1 binds with an ED50 of 12‑72 ng/mL.

Scientific Data Images for Recombinant Mouse CD200R1 His-tag Protein, CF

Recombinant Mouse CD200R1 His-tag Protein Binding Activity

Recombinant Mouse CD200R1 His-tag Protein Binding Activity

When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200 R1 (Catalog # 10054-CD) binds with an ED50 of 12-72 ng/mL.
Recombinant Mouse CD200R1 His-tag Protein SDS-PAGE

Recombinant Mouse CD200R1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD200 R1 His-tag (Catalog # 10054-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50 - 70 kDa.

Formulation, Preparation and Storage

10054-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1-3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a signal sequence, a 213 aa extracellular domain (ECD), a single transmembrane segment, and a cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of mouse CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5-7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13-15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

References

  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 
  8. Wright, G.J. et al. (2001) Immunology 102:173. 
  9. Hoek, R.M. et al. (2000) Science 290:1768.
  10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
  14. Zhang, S. et al. (2004) J. Immunol. 173:6786.
  15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.

Long Name

CD200 Receptor 1

Alternate Names

CD200 R1, MOX2R

Entrez Gene IDs

131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

Gene Symbol

CD200R1

UniProt

Product Documents for Recombinant Mouse CD200R1 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CD200R1 His-tag Protein, CF

For research use only

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