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Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF

Catalog # 10606-CV | R&D Systems, Inc. a Bio-Techne Brand
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10606-CV-100

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mers-cov Spike S1 Subunit protein
MERS-CoV Spike S1 Subunit
(Tyr18-Pro747)
Accession # K9N5Q8.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr18

Predicted Molecular Mass

107 kDa

SDS-PAGE

115-135 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human DPPIV/CD26 (High Purity Dimer) (Catalog # 9168-SE).

Scientific Data Images for Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF

Graph showing bioactivity of MERS Spike S1 Domain protein binding to Human DPPIV / CD26

Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein Bioactivity

Recombinant MERS-CoV Spike S1 Subunit Fc Chimera (Catalog # 10606-CV) binds Recombinant Human DPPIV/CD26 (High Purity Dimer) (9168-SE) in a functional ELISA.
SDS-PAGE gel of purified recombinant MERS Spike S1 Domain protein, Fc chimera

Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant MERS-CoV Spike S1 Subunit Fc Chimera (Catalog # 10606-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa and 220-260 kDa, respectively.
Flow cytometry scatter plot shows MERS Spike S1 Domain protein binds to CD26 / DPPIV expressing HEK293 cells

Detection of MERS-CoV Spike S1 Protein bound to CD26/DPPIV expressing cells by flow cytometry.

In a functional flow cytometry test, (A) Recombinant MERS-CoV Spike S1 Subunit Fc chimera Protein (Catalog # 10606-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD26/DPPIV and EGFP. Ligand binding was detected by staining cells with APC-conjugated anti-Human IgG Fc Monoclonal Antibody (FAB110A), which does not stain the cells in the absence of recombinant protein (B).

Formulation, Preparation and Storage

10606-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike S1 Subunit

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1,2). Other well-known human coronaviruses include several viruses that cause relatively mild respiratory disease, plus two viruses that caused the Severe Acute Respiratory Syndrome (SARS-CoV) and the global pandemic Covid-19 (SARS-CoV2). MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). Based on amino acid (aa) sequence homology, the MERS-CoV S1 subunit shares 23% and 22% identity with SARS-CoV S1 subunit and SARS-Cov2 S1 subunit, respectively. The low aa sequence homology is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). Unlike SARS-CoV and SARS-CoV2, which engage ACE2 as their receptors for cell entry, MERS-CoV employs Dipeptidyl Peptidase 4 (DPP4; also known as CD26) as its functional receptor (4). Based on structural biology studies, the receptor binding domain (RBD) of MERS-CoV spike protein is located in the C-terminal region of S1 subunit and consists of a core subdomain and a receptor-binding subdomain (5, 6). The S1 subunit, especially the RBD region, was commonly targeted for vaccinations or antiviral therapy against MERS (7-9).

References

  1. Bermingham, A. et al. (2012) Euro Surveill. 17:20290.
  2. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Lu, G. et al. (2013) Nature 500:227.
  6. Wang, N. et al. (2013) Cell. Res. 23:986.
  7. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  8. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  9. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.

Long Name

Spike Protein, S1 Subunit

UniProt

Product Documents for Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant MERS-CoV Spike S1 Subunit Fc Chimera Protein, CF

For research use only

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