Human IgG Fc APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB110A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Ovine
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 97924
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IgG Fc APC-conjugated Antibody
Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for Staining Membrane-associated Proteins.Applications for Human IgG Fc APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Human IgG Fc APC-conjugated Antibody
Product Specific Notices for Human IgG Fc APC-conjugated Antibody
For research use only
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