Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Name: Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein
Brand: R&D Systems
SKU: 375-TEC
Rating: 5 (1 reviews)

Catalog # 375-TEC | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (11)

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Key Product Details

Source:	E. coli
Accession #:	Q6IBA9
Structure / Form:	Homotrimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Technical Data
Citations (11) / Reviews (1)

Product Specifications for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Source	E. coli-derived human TRAIL/TNFSF10 protein Val114-Gly281, with and without an N-terminal Met
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val114
Predicted Molecular Mass	19 kDa (monomer)
SDS-PAGE	19 kDa (monomer)
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 0.5‑6 ng/mL. Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

375-TEC

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, (NH₄)₂SO₄ and ZnSO₄ with BSA as a carrier protein.
Reconstitution	Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 2 weeks, 2 to 8 °C under sterile conditions after reconstitution. 1 month, -20 to -70 °C under sterile conditions after reconstitution.

375-TEC/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, (NH₄)₂SO₄ and ZnSO₄.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 2 weeks, 2 to 8 °C under sterile conditions after reconstitution. 1 month, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL/TNFSF10

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is the most homologous to Fas ligand, sharing 28% aa sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% aa sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that transduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4). It was shown that homotrimeric TRAIL binds a zinc ion which is critical for the correct structure of the protein (5 - 6).

Item	Value
References	Golstein, P. (1997) Current Biology 7:R750. Wiley, S.R. et al. (1995) Immunity 3:673. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687. Emery, J. et al. (1998) J. Biol. Chem. 273:14363. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
Long Name	TNF-related Apoptosis-inducing Ligand
Entrez Gene IDs	8743 (Human); 22035 (Mouse); 246775 (Rat)
Alternate Names	APO2L, APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta, Apo-2 ligand, Apo-2Ltumor necrosis factor (ligand) family, member 10, CD253, CD253 antigen, Protein TRAIL, TL2, TNF-related apoptosis-inducing ligand, TNFSF10, TRAIL, TRAILTNF-related apoptosis inducing ligand TRAIL, tumor necrosis factor (ligand) superfamily, member 10, tumor necrosis factor ligand superfamily member 10

Citations for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein (11)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Frank-Bert et al. (2018-01-29), TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles Front Immunol, 2018-09(0):28.
PMID: 29434584
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Zoller et al. (2017-07-18), Trail (TNF-related apoptosis-inducing ligand) induces an inflammatory response in human adipocytes Sci Rep, 2017-07(1):5691.
PMID: 28720906
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-11-18), Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis Sci Rep, 2016-16(0):36862.
PMID: 27857173
Species: Human
Sample Types: Whole Cells
Applications: CAR-T (Bioassay)

Roybal K et al. (2016-09-29), Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell, 2016-0167(2):419-432.e16.
PMID: 27693353
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bozza et al. (2014-09-04), The use of a stably expressed FRET biosensor for determining the potency of cancer drugs. PLoS ONE, 2014-09(9):e107010.
PMID: 25188024
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Guicciardi et al. (2014-03-14), Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity. PLoS ONE, 2014-09(3):e92124.
PMID: 24633224
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Park SJ et al. (2009-04-28), Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP. Biochem. J., 2009-0420(1):73-81.
PMID: 19203346
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Roux S et al. (2005-07-22), Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis. Biochem. Biophys. Res. Commun., 2005-0333(1):42-50.
PMID: 15936719
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chopin V et al. (2004-08-15), Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1). Exp. Cell Res., 2004-0298(2):560-73.
PMID: 15265702
Sample Types: Whole Cells
Applications: Bioassay

Ryan LA et al. (2004-03-01), TNF-related apoptosis-inducing ligand mediates human neuronal apoptosis: links to HIV-1-associated dementia. J. Neuroimmunol., 2004-0148(1):127-39.
PMID: 14975593
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

Sade H et al. (2003-10-28), The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. J. Biol. Chem., 2003-1279(4):2937-44.
PMID: 14583609

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Customer Reviews for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein (1)

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Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Name: Anonymous

Application: Apoptosis assay

Verified Customer | Posted 12/16/2019

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Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Catalog # 375-TEC | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

375-TEC

Carrier

375-TEC/CF

Carrier Free

Reconstitution Calculator

Background: TRAIL/TNFSF10

Citations for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein (11)

Customer Reviews for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein (1)

Customer Images

Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Product Documents for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Product Datasheets

COA

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SDS

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